rt with anti cd44 e7k2y monoclonal antibody (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
rt with anti cd44 e7k2y monoclonal antibody
Rt With Anti Cd44 E7k2y Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt with anti cd44 e7k2y monoclonal antibody/product/Cell Signaling Technology Inc
Average 96 stars, based on 174 article reviews
Rt With Anti Cd44 E7k2y Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt with anti cd44 e7k2y monoclonal antibody/product/Cell Signaling Technology Inc
Average 96 stars, based on 174 article reviews
rt with anti cd44 e7k2y monoclonal antibody - by Bioz Stars,
2026-02
96/100 stars
Images
1) Product Images from "A conditionally replicative adenovirus vector containing the synNotch receptor gene for the treatment of muscle-invasive bladder cancer"
Article Title: A conditionally replicative adenovirus vector containing the synNotch receptor gene for the treatment of muscle-invasive bladder cancer
Journal: Cancer Gene Therapy
doi: 10.1038/s41417-025-00879-8
Figure Legend Snippet: Primer sequences for Real-time RT-PCR.
Techniques Used:
Figure Legend Snippet: a The expression of CD46, CAR, and CD44 on the surface of T24 cells was evaluated by flow cytometry. b The expression of CD46, CAR, and CD44 on the surface of BT474 cells was evaluated by flow cytometry. c The expression of CD46, CAR, and CD44 on the surface of KK47 cells was evaluated by flow cytometry. d The expression of CD46, CAR, and CD44 on the surface of 5637 cells was evaluated by flow cytometry. e The mRNA expressions of HAS1, HAS2 and HAS3 in KK47, 5637 and T24 cells were measured by real-time RT-PCR. f The mRNA expressions of COX-2 in KK47, 5637 and T24 cells were measured by real-time RT-PCR. All mRNA levels were standardized by the expression levels of the control gene TATA-binding protein (TBP) and analyzed using the ΔΔCt method. All these results were expressed as the mean ± SE for three separate experiments (* p < 0.05, ** p < 0.01).
Techniques Used: Expressing, Flow Cytometry, Quantitative RT-PCR, Control, Binding Assay
Figure Legend Snippet: The T24 cells were infected with ADX730 ( a ) at 100 MOI and CRAd-synNotch ( c ) at 50 MOI, and cultured for 24, 48, and 72 h. The expression of the synNotch receptor was analyzed by Western blotting using an anti-CD44 antibody. The T24 cells were infected with ADX730 ( b ) at 100 MOI and CRAd-synNotch ( d ) at 50 MOI, and cultured for 24, 48, and 72 h. The expression of the synNotch receptor was analyzed by Western blotting using a HIF-3α4 antibody. e The gene expression of HIF-3α4 in T24 cells was measured by real-time RT-PCR after Ad-lacZ and ADX730 infections. f The gene expression of HIF-3α4 in T24 cells was measured by real-time RT-PCR after CRAd-GFP and CRAd-synNotch infections. T24 ( g ) and BT474 ( h ) cells were treated with 50 MOIs of ADX730, CRAd-GFP, and CRAd-synNotch and incubated at 37°C for 72 h under oxygen concentrations of 2% (Hypoxia) or 21% (Normoxia). The absorbance was measured at a wavelength of 492 nm. All mRNA levels were standardized by the expression levels of the control gene TATA-binding protein (TBP) and analyzed using the ΔΔCt method. All these results were expressed as the mean ± SE for three separate experiments (** p < 0.01).
Techniques Used: Infection, Cell Culture, Expressing, Western Blot, Gene Expression, Quantitative RT-PCR, Incubation, Control, Binding Assay
